The squad is comprised of 15 Brumbies, seven Queensland Reds, six Waratahs, four Melbourne Rebels and three from Japanese-based clubs
NSW Waratahs pair Jed Holloway and David Porecki are amongst the six uncapped players in the squad as the Wallabies look for their first win over England since 2015.
They are joined by Brumbies locks Nick Frost and Cadeyrn Neville along with Rebels prop Pone Fa'amausili.
Reds flyer Suliasi Vunivalu has also earned a call-up after strong performances following his return from injury.
Allan Ala'alatoa (28, Brumbies, 53 Tests)
Tom Banks (27, Brumbies, 19 Tests)
Angus Bell (21, NSW Waratahs, 16 Tests)
Quade Cooper (34, Kintetsu Liners, 75 Tests)
Pone Fa'amausili (25, Melbourne Rebels, uncapped)
Folau Fainga'a (27, Brumbies, 25 Tests)
Nick Frost (22, Brumbies, uncapped)
Jake Gordon (28, NSW Waratahs, 10 Tests)
Michael Hooper (c) (30, NSW Waratahs, 118 Tests)
Jed Holloway (29, NSW Waratahs, uncapped)
Len Ikitau (23, Brumbies, 13 Tests)
Andrew Kellaway (26, Melbourne Rebels, 13 Tests)
Samu Kerevi (28, Suntory Sungoliath, 38 Tests)
Marika Koroibete (29, Saitama Wild Knights, 42 Tests)
Rob Leota (25, Melbourne Rebels, 6 Tests)
Noah Lolesio (22, Brumbies, 9 Tests)
Lachlan Lonergan (22, Brumbies, 4 Tests)
Tate McDermott (23, Queensland Reds, 15 Tests)
Cadeyrn Neville (33, Brumbies, uncapped)
James O’Connor (31, Queensland Reds, 61 Tests)
Hunter Paisami (24, Queensland Reds, 15 Tests)
Izaia Perese (25, NSW Waratahs, 2 Tests)
Jordan Petaia (22, Queensland Reds, 16 Tests)
Matt Philip (28, Melbourne Rebels, 20 Tests)
David Porecki (29, NSW Waratahs, uncapped)
Pete Samu (30, Brumbies, 19 Tests)
Scott Sio (30, Brumbies, 69 Tests)
James Slipper (33, Brumbies, 114 Tests)
Darcy Swain (24, Brumbies, 10 Tests)
Taniela Tupou (26, Queensland Reds, 38 Tests)
Rob Valetini (23, Brumbies, 18 Tests)
Suliasi Vunivalu (26, Queensland Reds, uncapped)
Nic White (31, Brumbies, 47 Tests)
Harry Wilson (22, Queensland Reds, 10 Tests)
Tom Wright (24, Brumbies, 10 Tests)